BBa_K1636000

BBa_K1636000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1636000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Roger Milton Rubio Sanchez, Ana Laura Torres

Date created: 2015-09-04 11:00:00

Date modified: 2015-09-05 06:51:22

Lead-II-ions-specific aptamer

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_K1636000_sequence (Version 1)

Description

This aptameric sequence is capable of recognising and binding specifically to lead II ions. This sequence, which works as a single stranded nucleic acid molecule, is capable of forming a G-quadruplex by interacting with lead ions (Pb2+) while disrupting a partially complementary duplex.

Notes

For standardisation purposes and its usage in iGEM, this sequence was cloned as a duplex on pSB1C3 thereby adding the prefix and suffix sequences to it.
Given the fact that the sequence is need as a single-strand molecule, a primer was designed in order to carry out a PCR with a single primer for the production of this molecule.

Source

This sequence does not come from a genomic sequence. First reported by Li et al 2010, it is comprised by 16 nucleotides with a high percentage of G which are the ones capable of stabilising and forming the quadruplex with the lead ion.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1636000/1

Member of these Collections

• //dna/aptamer
• iGEM Parts Registry