BBa_K1638037

BBa_K1638037 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1638037
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jens Sivk??r Pettersen
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 05:42:16

CsrA fused to T25 domain of CyaA with cAMP-induced RFP reporter



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1638037_sequence (Version 1)

Description

The carbon storage regulator CsrA fused to the T25-domain of the adenylate cyclase, CyaA, from Bordetella pertussis. This construct can be used in the two-hybrid screening of protein-protein interactions between CsrA and another x protein fused to the T18 domain. If a random set of peptide aptamers is fused to the T18-domain, one can screen for specific peptide aptamers against CsrA.

Notes

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Source

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igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1638037/1