Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nurgeldi Bazarov
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-16 11:20:08
LacI regulated DsRed with miR 26a and miR 375 binding sites
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1639009_sequence (Version 1) |
Description
bir onceki partimizdaki LacI bunu baskilaycaktir.... DsRed ise GFP nin bir derivative halidir. kirmizi floresan proteinidir.mustafa buraya resimleri eklemeyi bilmiyorum halledelim.
Notes
miRNA binding sites make it possible to supress its expression by increasing miR levels. But in gastric cancer cells the levels of miR26a and miR 375 are low unlikely of normal epithelial cells. As a result expression of this protein supressed in normal cells but not in cancerous cells.Source
This part is combination of lac operators derived from E.coli and DsRed protein originally found in Discosoma sp. and also this part contains miR binding sites at 3' end.| Sequence Annotation | Location | Component / Role(s) |
| lacO lacO (symmetric) Kozak DsRed ATG Stop Codon miR-26a binding site miR-375 binding site | 9,25 78,97 109,114 115,792 115,117 790,795 802,823 843,864 | feature/operator operator operator feature/operator feature/rbs ribosome_entry_site CDS feature/cds start_codon feature/start stop_codon feature/stop non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site |