Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pai Li
Date created: 2015-09-11 11:00:00
Date modified: 2015-09-16 11:16:17
Fusion protein of Vika-EGFP-ssra, with RBS at beginning
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1641009_sequence (Version 1) |
Description
This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Dre::EGFP::ssra-tag. The part is for expression of Vika-EGFP-ssra, which aims to overturn our invertase modules in our Micro-timer.Vika (BBa_K1641003) in our bricks is a Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel Vox sites up-side-down. An EGFP fusion at the C-term of Vika will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two parts to further avoid side-effect like misfolding. An ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression.
Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without start code and restriction enzyme activity. This sequence is compatible with brick construction method and usually less likely to make trouble.
Notes
Ssra-Tag: for efficiently cleaning the protein when not under induction, and helps to reduce leakage expression.Dre is a Cre-like recombinase, so C-term EGFP fusion will not significant reduce its activity.