BBa_K1641021

BBa_K1641021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-10 11:00:00

Date modified: 2015-09-11 10:09:26

Up-side-down RBS::mcherry-ssra(fast) with LoxP, recognition site of Cre

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region

Description

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two LoxP sites at the upstream and downstream of the gene.
LoxP can be recognized and the middle sequence can be restored by invertase Cre. Ssra-tag in this sequence is fast type (BBa_M0051) that significantly accelerates the degradation but at a speed less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without start code and restriction enzyme activity. This sequence is absolutely compatible with brick construction method and usually less likely to make trouble.

Notes

This part is constructed by PCR or cut-and-link of biobricks.

Source

This sequence is up-side-down, hence can be inverted into normal sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
conserved	5,8	conserved_region, feature/conserved
BBa_B0034	1,12	feature/BioBrick, engineered_region

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641021/1

Member of these Collections

• iGEM Parts Registry