BBa_K1646000

BBa_K1646000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1646000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jimin Lin

Date created: 2015-09-03 11:00:00

Date modified: 2015-09-04 08:19:23

EGFP-LC3

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1646000_sequence (Version 1)

Description

Our team decided to add LC3 as a new bio-brick into iGEM???s gene library. LC3, microtubule associated protein light chain 3, is a ubiquitin-like protein that binds to autophagosomes (AVs). If we transfect mammalian cells with GFP tagged LC3, we may be able to track and follow the fate of AVs in the cell and to measure autophagic flux.

Notes

EGFP linked at the N-terminal of LC3

Source

LC3 gene came from cDNA sequence of human.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1646000/1

Member of these Collections

• iGEM Parts Registry