Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yun Xue
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 11:22:23
B0034+ho1+B0034+pcyA+B0010
| Types | DnaRegion |
| Roles | Intermediate engineered_region |
| Sequences | BBa_K1647002_sequence (Version 1) |
Description
A set of enzymes that could synthesize phycocyanobilin in E. Coli under the control of strong promoter B0034. Ho1 is one member of the heme oxygenases family. It functions in producing BV IXa from endogenous heme in E. Coli. Ho1 catalyses stereospecific cleavage of heme and releases Fe2+ and carbon monoxide, which is the first step of phycocyanobilin synthesis. The second step is conducted by a phycocyanobilin:ferredoxin oxidoreductase (pcyA) which functions in reducing BV IXa.Notes
Used a strong RBS to produce high levels of expression of two enzymes for phycocyanobilin synthesis in E. Coli. Added a terminator for convenience.Source
We get this part from the 2014 DNA Distribution Kit.| Access | Instance | Definition |
| public public public public public | BBa_B0034 BBa_I15008 BBa_B0034 BBa_I15009 BBa_B0010 | BBa_B0034 BBa_I15008 BBa_B0034 BBa_I15009 BBa_B0010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_I15008 BBa_B0034 BBa_I15009 BBa_B0010 | 1,12 19,769 778,789 796,1570 1579,1658 | BBa_B0034 BBa_I15008 BBa_B0034 BBa_I15009 BBa_B0010 |