Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yun Xue
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 01:17:59
J23100+Cph8
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1647005_sequence (Version 1) |
Description
A functional red light sensor. It activates the expression of OmpC promoter when red light intensity is low while inhibits when red light intensity is higher than a threshold.The light-sensing protein we use is Cph8, a chimeric protein which produces strong response to light. It consist of a light-sensing domain Cph1 and an EnvZ domain. Phococyanobilin is necessary for Cph1 to response to light, but it???s not naturally produced in E Coli. Another two genes , ho1 and pcyA, can produce two enzymes that convert the endogenous haem into phococyanobilin. Functioning as a kinase, the EnvZ domain could lead to autophosphorylation of an endogenous regulator OmpR when light intensity remains below threshold. Those OmpR activate the ompC promoter. But when light intensity is high, autophosphorylation is inhibited and therefore, the expression stops.
Notes
Used a Cph8 protein, equipped with two assistant gene ho1 and pcyA, for strong response to red light.Source
We get this part from the 2014 DNA Distribution Kit| Access | Instance | Definition |
| public public | BBa_J23100 BBa_K909003 | BBa_J23100 BBa_K909003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_K909003 | 1,35 44,2298 | BBa_J23100 BBa_K909003 |