| Types | DnaRegion
|
| Roles | Device
engineered_region
|
| Sequences | BBa_K1650011_sequence (Version 1)
|
Description
This plasmid encodes a sRNA with 24 bp which is complementary to a sequence in the mRNA of MinC thus, leading to a prevention of its biosynthesis. Since the Min-system regulates symmetric cell division, this sRNA stimulates the formation of minicells which do not contain chromosomal DNA and therefore, are not able to propagate making them a chassis with a low risk in biosafety. The plasmid contains additionally a high-copy origin of replication and a cassette for the resistance against Chloramphenicol.
Notes
Sequence verification before usage to ensure correct sequence in order to use advanced cloning techniques.
Source
The basic idea for this device comes from the iGEM team Paris Bettencourt 2013.
