BBa_K165019

BBa_K165019 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K165019
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Szymanski
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:10:55

CFPx2 + SV40 NLS + ADH1 yeast terminator



    • Details

    Types
    DnaRegion

    Roles
    Intermediate

    engineered_region

    Sequences BBa_K165019_sequence (Version 1)

    Description

    This intermediate consists of two copies of CFP fused to the SV40 nuclear localization sequence, ending with the ADH1 terminator. It can be used to build synthetic transcription factors for use in yeast by ligating it to a construct containing Kozak+(repression/activation) domain + DNA-binding domain.

    Notes

    -

    Source

    We built this part using the CFPx2 gene from David Drubin, and the NLS+term part from Caroline Ajo-Franklin.

    Access Instance Definition
    public
    public
    		BBa_K165014
    BBa_K165018
    		cfpx2
    NLS+Term

    Sequence Annotation Location Component / Role(s)
    BBa_K165014
    BBa_K165018
    		1,1494
    1503,1755
    		cfpx2
    NLS+Term

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K165019/1
     

    Attachments

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