BBa_K165025

BBa_K165025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K165025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Szymanski

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:10:55

Kozak + Sin3 repression domain + LexA DNA-binding domain

Details

• Types: DnaRegion
• Roles: Intermediate; engineered_region
• Sequences: BBa_K165025_sequence (Version 1)

Description

This is the kozak sequence and Sin3 transcriptional repression domain ligated to the LexA DNA-binding domain. It can be used to build synthetic transcription repressors that bind to the lex operator by ligating it to (FPx2*)+(NLS+term)

Notes

-

Source

Constructed in our lab.

Components

Access	Instance	Definition
public	BBa_K165002	BBa_K165002
public	BBa_K165003	Sin3
public	BBa_K165009	lexA DBD

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K165002	1,18	BBa_K165002
BBa_K165003	27,2837	Sin3
BBa_K165009	2846,3103	lexA DBD

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K165025/1

Member of these Collections

• iGEM Parts Registry