BBa_K165060

BBa_K165060 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K165060
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aaron Glieberman
Date created: 2008-10-29 12:00:00
Date modified: 2015-05-08 01:10:56

pRS303 yeast shuttle vector, HIS3



Types
DnaRegion

Roles
Plasmid

plasmid

Sequences BBa_K165060_sequence (Version 1)

Description

Useful for chromosomal integration of a part into yeast strains.

R Daniel Gietz & Robert H Schiestl. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method . Nature protocols

Notes

As mentioned in the description of the part, this vector is currently not compatible with Standard Assembly.

Source

Original Sikorski vector was described in R. S. Sikorski and P. Hieter, Genetics, 122: 19-27 (1989).

Also refer to the sections on ???Strategy for Making Parts??? and ???Commonly Used Plasmids??? at http://openwetware.org/wiki/Silver:_Protocols#Synthetic_Biology.

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BBa_K165060/1