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BBa_K1652000

BBa_K1652000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1652000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Martin Hanzel

Date created: 2015-09-07 11:00:00

Date modified: 2015-09-08 12:39:58

pPGK1Gx

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Details

Types	DnaRegion
Roles	promoter Regulatory
Sequences	BBa_K1652000_sequence (Version 1)

Description

PGK1 promoter with two gal4 binding sites close to the transcription start site (TSS) that cause the promoter to be repressed in the presence of Gal4 binding protein.

Notes

TSS in PGK1 is not well-defined, and there exists a region at the end of the promoter, about 100 bp long, that is very AT-rich. The promoter also contains two elements that resemble TATA boxes.

Source

S. cerevisiae

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pPGK1 Gal4 site Gal4 site TATA? TATA?	1,872 740,756 758,774 724,729 864,870	feature/promoter promoter non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/misc sequence_feature feature/misc sequence_feature