BBa_K1658006

BBa_K1658006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1658006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Şeniz Y??ksel

Date created: 2015-09-14 11:00:00

Date modified: 2015-09-18 04:31:31

CAD1 Enzyme

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1658006_sequence (Version 1)

Description

Involved in lignin biosynthesis. Catalyzes the final step specific for the production of lignin monomers. Catalyzes the NADPH-dependent reduction of coniferaldehyde, 5-hydroxyconiferaldehyde, sinapaldehyde, 4-coumaraldehyde and caffeyl aldehyde to their respective alcohols

Notes

Gene name: CAD1
Length: 1315 bp
Vector name: pUC57

Sequence:
GAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTC
CTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGAT
GGGCTCGCTGGAAGTGGAACGTAAAACCGTGGGCTGGGCTGCTCG
TGACCCGTCTGGCGTGCTGTCCCCGTATGAATACACCCTGCGTAA
CACCGGCCCGCAGGATGTGTATGTTGAAGTCATGTGCTGTGGTAT
TTGTCATACGGATGTTCACCAAATCAAAAACGACCTGGGCATGAG
TAATTACCCGATGGTGCCGGGCCATGAAGTGGTTGGTGAAGTCGT
GGAAGTGGGCTCCGAAGTTACCAAATTTCGTGCCGGTGATGTTGT
CGGCGTCGGTTGTATTGTGGGCTCATGCGGTAACTGTCGCCCGTG
CAATTCGGATATTGAACAGTACTGCAACAAGAAAATTTGGAGTTA
CAACGATGTTTATCCGGATGGTAAACCGACCCAGGGCGGTTTTGC
AGGTGCTATGGTGGTTGATCAAAAATTCGTCGTGAAAATTCCGGA
TGGTATGGCCCCGGAACAGGCCGCCCCGCTGCTGTGCGCCGGTGT
GACGGTTTACAGCCCGCTGAATCATTTCGGCCTGAAACAATCTGG
TCTGCGCGGCGGTATCCTGGGTCTGGGCGGTGTTGGTCACATGGG
TGTGAAAATTGCGAAAGCGATGGGCCATCACGTCACCGTGATCAG
CTCTAGTGATAAAAAACGTGCAGAAGCTCTGGACCATCTGGGCGC
GGATGACTATCTGGTTTCCTCAGATGCAGCTCGCATGCAGGAAGC
GGCCGATTCTCTGGACTATATTATCGACACGGTTCCGGTCTTTCA
CCCGCTGGAACCGTACCTGAGCCTGCTGAAAATTGATGGCAAACT
GATCCTGATGGGTGTTGTCAACACCCCGCTGCAATTCGTTAGCCC
GATGGTCATGCTGGGTCGTAAATCTATTACGGGCAGTTTTATCGG
TTCCATGAAAGAACTGGCAGAAATGCTGGAATTTTGTAAAGAAAA
AGACCTGAGCAGCACCATCGAAATCGTGAAAATGGACTACATCAA
CACGGCTTTTGAACGCCTGGAAAAAAATGACGTGCGTTATCGCTT
CGTGGTGGATGTCGCGGGTAGCAAACTGTATCAATGATACTAGAG
CCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCT
TTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTC
ACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAG
TAGCGGCCGCTGCAG

Source

Ocimum basilicum is sweet basil (Eukaryota, Viridiplantae, Streptophyta, Embryophyta, Tracheophyta, Spermatophyta, Magnoliophyta, eudicotyledons, Gunneridae, Pentapetalae, asterids, lamiids, Lamiales, Lamiaceae,Nepetoideae, Ocimeae, Ocimum)
AY879285.1
Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.195) is an NADP(H) specific oxidoreductase catalysing the reversible conversion of cinnamyl aldehydes to the corresponding alcohols or monolignols (Mansel et al., 1974; Wyrambik and Griesebach, 1975; Boudet et al., 1995). Polymerization of the three monolignols: /?-coumaryl, sinapyl and coniferyl alcohols lead to lignin. The enzyme is closely related to lignification, and chemical inhibition of its activity reduces the synthesis of lignins (Moesbacher et al., 1990; Mauch-Mani and Slusarenko, 1996).

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1658006/1

Member of these Collections

• iGEM Parts Registry