BBa_K1660003

BBa_K1660003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1660003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dai Yuanyi
Date created: 2015-09-06 11:00:00
Date modified: 2015-09-07 10:42:36

d-limonene synthase



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1660003_sequence (Version 1)

Description

This part can express d-limonene synthase with a constitutive promoter J23100, can it can catalyze GPPs into d-limonenen. We use this part to produce d-limonene which is said can attract nematode and some other pests well.

Notes

We searched the cDNA sequence in NCBI, and optimized the sequence for E. coli. Meanwhile, we inserted a Xho I restriction site between RBS and d-limonene synthase coding sequence, so that we can change promoter easily.

Source

It comes from Citrus unshiu CitMTSE1 d-limonene synthase.

Sequence Annotation Location Component / Role(s)
J23100
B0032
start codon
d-limonene synthase
stop codon
1,35
44,46
63,65
63,1883
1881,1883
promoter feature/promoter
ribosome_entry_site feature/rbs
feature/start start_codon
CDS feature/cds
stop_codon feature/stop
igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1660003/1