BBa_K1669001

BBa_K1669001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1669001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Marina Klemencic

Date created: 2015-09-11 11:00:00

Date modified: 2015-09-12 01:47:27

CtfA + His-tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1669001_sequence (Version 1)

Description

This part includes the CtfA gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli.

This is one of the two polipeptide chains forming the CtfAB (CoA-transferase) enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyrate to butyril-CoA.

The sequence contains a C-terminal His-tag for purification of the protein using the mmobilized-metal affinity chromatography and detected with the anti-His antibodyes.

Notes

Codon optimization for expression in E. coli.
Added C-terminal His-tag.

Source

Sequence retrieved from GenBank and codon optimized for expression in E. coli.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG	1,3	feature/start, start_codon
double stop codon	669,673	stop_codon, feature/stop
His-tag	650,668	feature/tag, tag
CtfA	1,649	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1669001/1

Member of these Collections

• iGEM Parts Registry