Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: JIAQI XU
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-15 09:57:31
NhaB
sodium-proton antiporter
membrane protein
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1675001_sequence (Version 1) |
Description
The gene, NhaB, is the archetypal sodium-proton antiporter in E. coli. It is crucial when the level of NhaA activity is growth limiting, when NhaA is not sufficiently induced, or when NhaA is not activated.NhaA catalyzes the exchange of 3 proton ion per 2 sodium ion. Therefore, NhaA could make sure E. coli???s survival under alkaline pH, which are in desperate need in our project.
Notes
mutation of CTG to CTA Leu.(site:336bp); mutation of GCT to GCG Ala.(site:414bp); mutation of ACC to ACT Thr.(site:519bp); mutation of GAC to GAT Asp.(site:525bp); mutation of ACC to ACT Thr.(site:630bp); mutation of GTA to GTG Val.(site:645bp); mutation of GCA to GCG Ala.(site:993bp); mutation of GAG to GAA Glu.(site:1020bp); mutation of GTC to GTA Val.(site:1452bp)Source
Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=1233176&to=1234717&strand=true| Sequence Annotation | Location | Component / Role(s) |
| NhaB Coding Region ATG start codon TGA stop codon | 1,1542 1,3 1540,1542 | CDS feature/cds start_codon feature/start feature/stop stop_codon |