Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: JIAQI XU
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 12:38:27
FimE recombinase device
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1675010_sequence (Version 1) |
Description
FimE is different from other kind???s recombinase. Its recognized sites on the upstream (IRL) and downstream (IRR) are not same. It means that after the recombination mediated by FimE happens, the sign sites would be exchanged, so that the new sign sites couldn???t be recognized by FimE again. So the recombination mediated by FimE is irreversible. [17]Granted that the bacteria produce the strong alkali in the primary, the environment would turn alkaline quickly. So P-atp2 can correspond the change of environment and start the expression of FimE. FimE recognizes IRL and IRR, then catalyze the inversion of J23119. The weak acid would be generated at that time. For the environment, due to the production of the weak acid, it would turn neutral gradually.
Since the recombinases were found and RMCE was invented, more recombinases??? functions and features were be explored. Recombinase has stick logic relationship and high robust. It fits the modern research of synthetic biology.
Notes
None.Source
P-atp2 is from the standard part BBa_K1675016. http://parts.igem.org/Part:BBa_K1675016The RBS is from the standard part BBa_B0034. http://parts.igem.org/Part:BBa_B0034
Gene FimE is from the standard part BBa_K137007. http://parts.igem.org/Part:BBa_K137007
| Access | Instance | Definition |
| public public public | BBa_K1675016 BBa_B0034 BBa_K137007 | BBa_K1675016 BBa_B0034 BBa_K137007 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1675016 BBa_B0034 BBa_K137007 | 1,607 616,627 634,1191 | BBa_K1675016 BBa_B0034 BBa_K137007 |