BBa_K1675017

BBa_K1675017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1675017
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jing Yang
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-16 07:34:18

P-atp2 mutant 430-B0034-lacz alpha



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1675017_sequence (Version 1)

Description

The natural P-atp2 is an alkali-induced promoter in Corynebacterium glutamicum located in F0F1 ATPase operon. The natural P-atp2 promoter responds to the pH changes from pH 7.0 to pH 9.0, especially at alkaline pH. When the pH value increasing, the expression increased modestly. It is activated by the alternative sigma factor of the RNA polymerase, whose synthesis would be activated when the bacteria are growing at alkaline pH.

The activity of this mutant at different pH is shown in figure 1.

Notes

There is an EcoRI site exists in the P-atp2 sequence, so we mutate it to change it.

Source

Error prone PCR of P-atp2, then overlap extension of synthetic oligonucleotides.

The natural P-atp2 is from Corynebacterium glutamicum.

Lacz alpha is from http://parts.igem.org/wiki/index.php?title=Part:BBa_I732006

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1675017/1