Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jing Yang
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-16 07:37:33
P-atp2 mutant 705-B0034-lacz alpha
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1675021_sequence (Version 1) |
Description
The natural P-atp2 is an alkali-induced promoter in Corynebacterium glutamicum located in F0F1 ATPase operon. The natural P-atp2 promoter responds to the pH changes from pH 7.0 to pH 9.0, especially at alkaline pH. When the pH value increasing, the expression increased modestly. It is activated by the alternative sigma factor of the RNA polymerase, whose synthesis would be activated when the bacteria are growing at alkaline pH.The activity of this mutant at different pH is shown in figure 1.
Notes
There is an EcoRI site exists in the P-atp2 sequence, so we mutate it to change it.Source
Error prone PCR of P-atp2, then overlap extension of synthetic oligonucleotides.The natural P-atp2 is from Corynebacterium glutamicum.
Lacz alpha is from http://parts.igem.org/wiki/index.php?title=Part:BBa_I732006