BBa_K1675024

BBa_K1675024 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K1675024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jing Yang
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-16 09:37:28

P-atp2 mutant 389-B0034-lacz alpha



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1675024_sequence (Version 1)

Description

The natural P-atp2 is an alkali-induced promoter in Corynebacterium glutamicum located in F0F1 ATPase operon. The natural P-atp2 promoter responds to the pH changes from pH 7.0 to pH 9.0, especially at alkaline pH. When the pH value increasing, the expression increased modestly. It is activated by the alternative sigma factor of the RNA polymerase, whose synthesis would be activated when the bacteria are growing at alkaline pH.

The activity of this mutant at different pH is shown in figure 1.

Notes

There is an EcoRI site exists in the P-atp2 sequence, so we mutate it to change it.

Source

Error prone PCR of P-atp2, then overlap extension of synthetic oligonucleotides.

The natural P-atp2 is from Corynebacterium glutamicum.

Lacz alpha is from http://parts.igem.org/wiki/index.php?title=Part:BBa_I732006

igem#experience
None
 
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1675024/1