Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mackenzie Labine-Romain
Date created: 2015-08-20 11:00:00
Date modified: 2015-08-20 11:14:48
pCPC
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
Description
This integrative plasmid facilitates transformation of Synechocystis PCC6803 and similar species. It inserts gene of choice into the Synechocystis cpc gene by homologous recombination.Notes
Primers containing biobrick prefix and suffix as well and other described parts were used to make this backbone RFC10 compatible.Source
Plasmid was purchased from open source plasmid repository Addgene.com (originally named cpc-PHLS (g)). It was modified to be biobrick compatible.Formighieri, C., & Melis, A. (2014). Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta, 240(2), 309-324.