Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Barthelemy Caron
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-18 05:48:51
RibT promoted by p48 synthetic promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1678003_sequence (Version 1) |
Description
Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase.Coding sequence has been codon optimized for Lactobacillus plantarum.
Protein is expressed by a L.plantarum synthetic promoter p48 BBa_K1678004.
The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).
The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).
The expressed protein catalyses one step in the riboflavin pathway.
Notes
niobeSource
CDS is coming from B.subtilis genome and has been codon optimized for L.plantarum on IDT website| Sequence Annotation | Location | Component / Role(s) |
| RbT BBa_K1678004 Tldh terminator RBS | 96,470 21,80 471,608 81,95 | feature/cds CDS feature/promoter promoter sequence_feature feature/misc feature/rbs ribosome_entry_site |