BBa_K1678005

BBa_K1678005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1678005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Antoine Vigouroux

Date created: 2015-09-15 11:00:00

Date modified: 2015-09-16 10:55:53

Promoter from BBa_J23119 followed by 4 orthogonal LoxP sites

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1678005_sequence (Version 1)

Description

This parts contains the strong constitutive promoter of BBa_J23119, followed by four LoxP sites:
- LoxP
- Lox2272
- LoxN
- Lox5171
These LoxP sites are not supposed to recombine together. This biobrick may be useful for the construction of Brainbow-like systems.

Notes

Spacer have been added between the LoxP sites to allow for primer binding.

Source

DNA synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Bba_J23119 promoter	1,35	promoter, feature/promoter
LoxP	44,77	sequence_feature, feature/misc
Lox2272	86,119	feature/misc, sequence_feature
LoxN	132,165	sequence_feature, feature/misc
Lox5171	175,208	sequence_feature, feature/misc

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1678005/1

Member of these Collections

• iGEM Parts Registry