BBa_K1679034

BBa_K1679034 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1679034
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xihan Zhang
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 11:51:29

pT7-10



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1679034_sequence (Version 1)

Description

The four T7 RNA polymerases are a family with different activity, this T7 RNA polymerase have approximate relative activity of 4% compare to the wild type pT7. We design it as a part of our T7 protein expression device, which is high level and need low-activity promoter to exclude bacterial stress responses. Users can replace the T7 promoter with it or other promoters through the restriction site we added.

Notes

It's a member of T7 promoter mutations which is design for our T7 protein expression device and can be substitute by the restriction site HindIII and SalI.

Source

Synthesized by Genwiz.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1679034/1