BBa_K1679038

BBa_K1679038 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1679038
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xihan Zhang
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 01:15:04

RiboJ



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1679038_sequence (Version 1)

Description

After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme and an additional 23-nt hairpin immediately downstream to help expose the RBS. STRSV can cleaves the mRNA autocatalytically at a defined residue, which had the effect of cleaving extraneous RNA leaders that arose from transcription from promoters with different start sites.[1] Attributed to the biochemical function mentioned above, RiboJ has insulating capability. We use it to buffer synthetic circuits from genetic context.

[1] Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.

Notes

After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme and an additional 23-nt hairpin. Users can insert it into promoter and RBS to reduce noise caused by upstream sequence and stabilize mRNA.

Source

Synthesized by Genewiz.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1679038/1