BBa_K1680019

BBa_K1680019 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1680019

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nicolai von Kuegelgen

Date created: 2015-09-16 11:00:00

Date modified: 2016-01-26 10:15:55

Cre-dronpa fusion

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1680019_sequence (Version 1)

Description

Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 12 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.

Notes

3A assembly using RFC25

Source

gBlocks designed by Team Tuebingen

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
K1680002	1,30	feature/cds, CDS
K1680007	31,1059	feature/cds, CDS
K1680002	1060,1095	CDS, feature/cds
K1680006	1096,1764	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1680019/1

Member of these Collections

• //cds
• //cds/reporter/chromoprotein
• //cds/reporter/gfp
• //chassis/eukaryote/yeast
• //function/recombination/cre
• //function/reporter/fluorescence
• iGEM Parts Registry