| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1680023_sequence (Version 1)
|
Description
Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 12 AA linker as spacer (BBa_K1680002). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
Notes
3A assembly using RFC25
Source
gBlock design by Team Tuebingen
