| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1683005_sequence (Version 1)
|
Description
The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Pseudomonas aeruginosa PA01. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump.
Notes
The main consideration when designing the signaling sequence and Pseudomonas aeruginosa PA01 TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.
Source
The tolC signaling sequence and Pseudomonas aeruginosa PA01 tolC sequence were synthesized from DNA sequences found in genomic databases.
