dTomato

BBa_K1688010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1688010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dianna Zeleskov
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-18 11:41:03

dTomato (inc RBS, J23101 promoter)



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1688010_sequence (Version 1)

Description

The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths do not overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. The emission peak is at 581 nm and the 50% emission peak is at 629 nm. Characterization has been made with the Anderson promoters BBa_J23101 (this construct) and BBa_J23110 (construct BBa_K1688011)

Source

dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed.

Access Instance Definition
public
public
BBa_J23101
BBa_K1688004
BBa_J23101
dTomato
igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1688010/1