BBa_K1688017

BBa_K1688017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1688017
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mikael Andersson Sch??nn
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-18 11:17:38

Nah7 - Mutagenized upper naphthalene breakdown pathway (inc RBS)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1688017_sequence (Version 1)

Description

Mutagenized upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida + RBS B0034. Operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate.

Notes

The pathway contained two endogenous restriction sites for the enzyme PstI which have been removed through site directed mutagenesis.

Source

Nah7 plasmid from the PpG7 strain of Pseudomonas putida. Standard iGEM registry RBS.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1688017/1