Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_K1688017_sequence (Version 1)
|
Description
Mutagenized upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida + RBS B0034. Operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate.
Notes
The pathway contained two endogenous restriction sites for the enzyme PstI which have been removed through site directed mutagenesis.
Source
Nah7 plasmid from the PpG7 strain of Pseudomonas putida. Standard iGEM registry RBS.