| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1688021_sequence (Version 1)
|
Description
ModLac is a modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence.
Notes
The gene is followed by a KpnI restriction site with glycines and serines on each side, which are small amino acids which increase the flexibility of the protein. The gene was originally synthesized with a C-terminus export tag attached. This tag was removed through digestion with Kpn1 restriction enzyme and resulted in this sequence.
Source
D439A/M510L CueO mutant sequence received from Professor Kunishige Kataoka and synthesized with His-tag.
