BBa_K1689014

BBa_K1689014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1689014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei weijia
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-27 01:31:12

dCas9-Clact



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1689014_sequence (Version 1)

Description

pSB1C3-6H-dCas9-Clact, C terminal segment of beta-lacatamse fused with dCas9, His tag allows for protein purification

Notes

delta alpha segment of LacZ fused with dCas9, His tag allows for protein purification

Source

E.coli

Sequence Annotation Location Component / Role(s)
6??His tag
dCas9
GGGGS*2 Linker
C terminal of β-Lactamase
dCas9-Clact
random spacer
start
stop
random spacer
37,54
55,4155
4156,4185
4186,4458
34,4461
1,33
34,36
4459,4461
4462,4532
tag feature/tag
feature/protein CDS
CDS feature/protein
CDS feature/protein
feature/cds CDS
feature/misc sequence_feature
feature/start start_codon
feature/stop stop_codon
sequence_feature feature/misc
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1689014/1