Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei weijia
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-27 01:35:06
dCas9-F[1,2]
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1689016_sequence (Version 1) |
Description
pSB1C3-6His-dCas9-F[1,2], this part is a fusion protein of dCas9-F[1,2], the His tag is used for protein purificationNotes
dCas9 fusion protein coding sequence is too long, so you'd better amplify it separately.Source
E.coli| Sequence Annotation | Location | Component / Role(s) |
| F[1,2]-dCas9 His tag dCas9 GGGGS*2 Linker F[1,2] random spacer start stop random spacer | 34,4449 37,54 55,4155 4156,4185 4186,4446 1,33 34,36 4447,4449 4450,4520 | CDS feature/cds feature/tag tag feature/protein CDS feature/protein CDS feature/protein CDS sequence_feature feature/misc start_codon feature/start stop_codon feature/stop sequence_feature feature/misc |