Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei weijia
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-27 09:51:28
dCas9-F3
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1689018_sequence (Version 1) |
Description
pSB1C3-6H-dCas9-F3, this is the fusion protein of dCas9-F3, His tag allows for protein purificationNotes
The coding sequence of this fusion protein is too long, you'd better amplify is separately.Source
E.coli| Sequence Annotation | Location | Component / Role(s) |
| dCas9-F3 His dCas9 GGGGS*2 Linker F3 random spacer start stop random spacer | 34,4401 37,54 55,4155 4156,4185 4186,4398 1,33 34,36 4399,4401 4402,4472 | feature/cds CDS tag feature/tag CDS feature/protein CDS feature/protein CDS feature/protein feature/misc sequence_feature feature/start start_codon feature/stop stop_codon sequence_feature feature/misc |