Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yue Liu
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 11:16:51
Riboflavin producing parts
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1696011_sequence (Version 1) |
Description
The riboflavin biosynthesis genes ribABDEC from E. coli are overexpressed under the control of the inducible trc promoter. Enzymes encoded by the genes shown are: ribA, GTP cyclohydrolase II; ribB, 3,4-dihydroxy-2-butanone 4-phosphate synthase; ribD, fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/ 5-amino-6-(5-phosphoribosylamino)uracil reductase; ribE, 6,7-dimethyl-8-ribityllumazine synthase; ribC, riboflavin synthase gene.Notes
We need to avoid some special enzyme site in the sequence.Source
E.coli| Sequence Annotation | Location | Component / Role(s) |
| trp promoter RBS-A ribA RBS-B ribB RBS-D ribD RBS-E ribE RBS-C ribC | 1,30 70,96 97,687 688,715 716,1369 1376,1410 1402,2505 2512,2544 2545,3015 3016,3050 3051,3692 | promoter feature/promoter ribosome_entry_site feature/rbs feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS feature/rbs ribosome_entry_site CDS feature/cds ribosome_entry_site feature/rbs feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS |