BBa_K1698003

BBa_K1698003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1698003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brandon Malone
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 06:58:48

BaseHunter : Activated to become detector for 32bp target sequence on SRY Gene



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K1698003_sequence (Version 1)

Description

Once digested with the four enzymes specified in our team's protocols, this part can be used to differenciate betwwen male and female mammalian DNA

Notes

We had to make sure a target was used which didn't contain any illegal sites for biobricks. Also had to make sure target would not form hairpin structures or have a high GC content

Source

Part was ordered from IDT and cloned into a BBa_K584001 plasmid backbone

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1698003/1