Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Roulier, Chauncy Hinshaw, Krista Henderson
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 02:58:57
Overexpression of E. coli fadL under control of lac promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1702001_sequence (Version 1) |
Description
Long chain fatty acids (LCFAs) are naturally transported into E. coli by a long-chain fatty acid outer membrane porin, which is encoded for by fadL. fadL and fadD are required for the uptake and break down of fatty acids in E. coli. This gene is under the control of the lac promoter.Notes
This plasmid allows for induced expression of fadL with lactose or IPTG.Source
The lac gene is sourced from the iGEM distribution kit. The fadL gene was extracted by genomic PCR from DH5alpha E. coli.| Access | Instance | Definition |
| public public | BBa_K861500 BBa_J04500 | BBa_K861500 BBa_J04500 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K861500 BBa_J04500 | 1,1341 1350,1569 | BBa_K861500 BBa_J04500 |