BBa_K1702001

BBa_K1702001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1702001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anthony Roulier, Chauncy Hinshaw, Krista Henderson

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 02:58:57

Overexpression of E. coli fadL under control of lac promoter

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1702001_sequence (Version 1)

Description

Long chain fatty acids (LCFAs) are naturally transported into E. coli by a long-chain fatty acid outer membrane porin, which is encoded for by fadL. fadL and fadD are required for the uptake and break down of fatty acids in E. coli. This gene is under the control of the lac promoter.

Notes

This plasmid allows for induced expression of fadL with lactose or IPTG.

Source

The lac gene is sourced from the iGEM distribution kit. The fadL gene was extracted by genomic PCR from DH5alpha E. coli.

Components

Access	Instance	Definition
public	BBa_K861500	BBa_K861500
public	BBa_J04500	BBa_J04500

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K861500	1,1341	BBa_K861500
BBa_J04500	1350,1569	BBa_J04500

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1702001/1

Member of these Collections

• iGEM Parts Registry