Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Astrid Deryckere
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-18 09:28:45
CheZ-GFP
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1709002_sequence (Version 1) |
Description
Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.Notes
1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid.CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
Source
DNA sequences originate from the E. Coli genome and previous biobrick sequences.| Sequence Annotation | Location | Component / Role(s) |
| ATG Start Codon Ochre Stop Codon CheZ Coding Region GFP Coding Region LVA-tag | 1,3 1399,1401 1,642 646,1365 1366,1401 | start_codon feature/start stop_codon feature/stop feature/cds CDS CDS feature/cds tag feature/tag |