BBa_K1713000

BBa_K1713000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1713000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xudong Luo
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-18 10:41:11

Plac/ara-1, a hybrid promoter



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1713000_sequence (Version 1)

Description

The Plac/ara-1 is a hybrid promoter which is composed of the activation operator site from the araBAD promoter and repression operator sites from the lacZYA promoter. And the araBAD promoter is placed in its normal location relative to the transcription start site while the lacZYA promoter is placed both upstream and immediately downstream of the transcription start site. In the meanwhile, It can be activated by the AraC protein in the presence of arabinose and repressed by the LacI protein in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).

Notes

N/A

Source

Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.

Sequence Annotation Location Component / Role(s)
Hybrid promoter
1,102
promoter feature/promoter
igem#experience Works  
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K1713000/1