Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xudong Luo
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-18 10:41:11
Plac/ara-1, a hybrid promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1713000_sequence (Version 1) |
Description
The Plac/ara-1 is a hybrid promoter which is composed of the activation operator site from the araBAD promoter and repression operator sites from the lacZYA promoter. And the araBAD promoter is placed in its normal location relative to the transcription start site while the lacZYA promoter is placed both upstream and immediately downstream of the transcription start site. In the meanwhile, It can be activated by the AraC protein in the presence of arabinose and repressed by the LacI protein in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).Notes
N/ASource
Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.| Sequence Annotation | Location | Component / Role(s) |
| Hybrid promoter | 1,102 | promoter feature/promoter |