Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hiroki ONO
Date created: 2015-09-09 11:00:00
Date modified: 2016-02-03 10:45:45
pBad/araC-RBS B0034-Ag43 thanatin as alpha domain-double terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1714000_sequence (Version 1) |
Description
Ag43 is a kind of outer membrane protein that is composed of 3 domains. First one is signal peptide that transport Ag43 to outer membrane. Second one is alpha domain that has connection peptide to another Ag43 alpha domain. Last one is beta domain that has beta barrel structure to let the barrel there. This part has thanatin, a kind of antimicrobial peptide as alpha domain. This thanatin has Asp as the last residue. So if thanatin domain is digested by Asp-N endoproteinase (NEB), this part secret thanatins.Notes
This part has 6 PstI sites so you shoud not digest this part by PstI.Source
This part is derived from BBa_K759001 and it contains thanatin-Asp fragment that is synthesized by IDT.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1714000 BBa_I0500 AraC PC Pbad RBS B0034 Ag43 Signal Peptide Thanatin as alpha domain Ag43 beta domain double terminator BBa_B0034 BBa_B0015 | 1,3074 1,1210 1,1079 1043,1114 1080,1210 1219,1230 1237,1395 1402,1467 1474,2945 2946,3074 1219,1230 2946,3074 | engineered_region feature/BioBrick feature/BioBrick engineered_region feature/misc sequence_feature feature/promoter promoter feature/promoter promoter feature/rbs ribosome_entry_site feature/protein CDS CDS feature/protein CDS feature/protein stem_loop feature/stem_loop engineered_region feature/BioBrick feature/BioBrick engineered_region |