Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Our Lady of the Snows Catholic Academy 2015
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-18 10:41:04
Keratinase US under control of LacI promoter.
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1717173_sequence (Version 1) |
Description
This part is a composite of an IPTG-inducible promoter with RBS (BBa_J04500) and a custom-synthesized KERATINASE US expressing plasmid. The coding sequence for Keratinase US was originally isolated in Bacillus, we have now optimized for expression in E.coli. To do this, the signal peptide has been changed.Notes
The sequence was form Bacillus, a gram-positive bacteria, and in order for the Keratinase to be secreted properly in E.coli (a gram-negative bacteria), the signal peptide had to be changed.Source
The source for the KerUS coding sequence is :http://www.uniprot.org/uniprot/A0A059PWB6. The IPTG promoter was found on the registry.| Access | Instance | Definition |
| public public | BBa_J04500 BBa_K1717172 | BBa_J04500 KERUS |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K1717172 | 1,220 229,1347 | BBa_J04500 KERUS |