| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1725420_sequence (Version 1)
|
Description
UirR is a protein that attaches itself to the transmembrane protein UirS (K1725410). After exposure to unidirectional UV-A, UirS releases UirR, which physically interacts with the lsiR promoter (K1725400), activating transcription. All three parts are required for a cell to respond to the UV-A stimulus.
Notes
Due to the length of the DNA sequence, the gene was amplified via PCR and the BioBrick suffix and prefix were added as part of the primers. The last base of the gene was changed from G to A because different stop codons can be more efficient in different species and TAA is especially efficient in E. coli.
Source
The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803
