BBa_K1725428

BBa_K1725428 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1725428
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrey Filipov
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-16 07:37:03

J23114 + RBS + UirR + B0015



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1725428_sequence (Version 1)

Description

The UV intensity response regulator coding sequence was positioned downstream of J23114, a constitutive promoter family member, and upstream of the double terminator B0015. This allows the part to be placed in front of other parts with weaker or non-constitutive promoters.

Notes

The regulator and J23114 were ligated to B0015 after restriction that was followed by an Azure-A-based gel extraction.

Source

The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1725428/1