Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: The Newcastle 2009 iGEM team
Date created: 2009-10-09 11:00:00
Date modified: 2015-05-08 01:10:58
SmtA metallothionein protein with CotC and Gfp fusion
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K174008_sequence (Version 1) |
Description
Metallothionein protein can bind to heavy metals such as cadmium, arsenic, mercury, silver. By fusing CotC spore coat protein from Bacillus subtilis, it can be localized to the spore coat, hence trap the metals into bacterial spores. It is also fused with Gfp to see the spores with the proteins using a microscope.Notes
It is integrated to B. subtilis using the homology between cotC fusion and the native copy of cotC on the chromosome.Source
smtA sequence is taken from E. coli| Sequence Annotation | Location | Component / Role(s) |
| BBa_K174009 (cotC) Gfp without start codon BBa_K174019 (smtA without start codon) terminator sigK promoter + rbs | 208,405 405,1116 1117,1284 1285,1318 1,207 | engineered_region feature/BioBrick feature/cds CDS engineered_region feature/BioBrick sequence_feature feature/misc promoter feature/promoter |