BBa_K174010

BBa_K174010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K174010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: The Newcastle 2009 iGEM team

Date created: 2009-10-12 11:00:00

Date modified: 2015-05-08 01:10:58

KinA

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K174010_sequence (Version 1)

Description

Spo0A is the master regulator of sporulation in B. subtilis. When it is phosphorylated, it activates the expression of more than 100 genes. It has been shown that, activation of spo0A is sufficient to trigger sporulation.

By artifically synthesising KinA during growth, spoulation can be triggered in a multicomponent phosphorelay in which KinA phosphorylates Spo0F, Spo0F~P in turn phosphorylates Spo0B, and finally Spo0B~P phosphorylates Spo0A.

Notes

KinB, KinC kinases are also effective to trigger sporulation. However we only designed our system using KinA.

Source

B. subtilis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
KinA	1,1818	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K174010/1

Member of these Collections

• iGEM Parts Registry