BBa_K174012

BBa_K174012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K174012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: The Newcastle 2009 iGEM team

Date created: 2009-10-13 11:00:00

Date modified: 2015-05-08 01:10:58

sleB, Bacillus subtilis germination gene with RBS

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K174012_sequence (Version 1)

Description

sleB encodes for a spore cortex-lytic enzyme. This enzyme responds germination triggered by L-alanine which is the most universal germinant for spores. It has been shown that B. subtilis mutants with inactivated sleB gene were not able to germinate (1).

Hence by regulating this gene in sleB mutant B. subtilis cells, germination rate can be contolled.

Notes

It has been added as a part with its own natural RBS.

Source

B. subtilis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	1,5	ribosome_entry_site, feature/rbs
SleB CDS	15,932	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K174012/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry