Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: The Newcastle 2009 iGEM team
Date created: 2009-10-13 11:00:00
Date modified: 2015-07-24 01:17:17
CadA promoter with CzrA binding site
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K174017_sequence (Version 1) |
Description
In B. subtilis CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by CzrA repressor protein. When certain metals get inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system.By taking the promoter of CadA system, we created a generic metal sensor. Hence when it is combined with coding sequences of other genes, it will regulate the expression of these genes upon sensing metals.
Notes
It was developed mainly to use combinatorial approaches for metal sensingSource
It is taken from B. subtilis cadA promoter.| Sequence Annotation | Location | Component / Role(s) |
| cadA promoter sigA -35 sigA -10 CzrA binding site | 11,144 18,23 42,47 56,86 | feature/promoter promoter non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/operator operator |