BBa_K1750000

BBa_K1750000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1750000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Janelle San Juan

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 12:30:29

loxP-flanked RFP upstream from NPTII

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1750000_sequence (Version 1)

Description

Red fluorescent protein (encoded by EforRED), which produces red light, is flanked by loxP sites that are recombined by Cre-recombinase to excise the intervening gene. The kanamycin-resistance gene NPTII is positioned downstream. This part should be used in conjuction with Cre-recombinase as a reporter for its activity.

In the absence of Cre-recombinase expression, RFP is expressed, thus cells appear red. Upon induction of Cre-recombinase expression, loxP recombination excises the EforRED gene, allowing expression of the kanamycin-resistance gene, therefore cells can grow on kanamycin-supplemented media.

Separate ribosomal binding sites and a terminator following RFP allow independent expression of both genes. This construct is under the control of the T7 promoter.

Notes

Incorporation of a terminator sequence downstream of RFP to reduce leaky read-through of NPTII (though leaky read-through still occurs with this construct).

Source

Multispecies

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1750000/1

Member of these Collections

• iGEM Parts Registry