I-SceI RS

BBa_K175027 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K175027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tim Weenink

Date created: 2009-07-31 11:00:00

Date modified: 2015-05-08 01:10:59

I-SceI restriction site

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_K175027_sequence (Version 1)

Description

I-SceI homing endonuclease site

Restriction site for the homing endonuclease I-SceI.

Sequence will be cut as follows:

5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3'

3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5'

Notes

Design was very simple. The recognition site was copied from NEB and synthesised

Source

synthesised

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
I-SceI homing endonuclease restriction site	1,30	feature/dna, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K175027/1

Member of these Collections

• //dna/restrictionsite
• iGEM Parts Registry